GlyGen Webinar Series
The GlyGen project organizes public webinars on diverse topics, ranging from bioinformatics databases to new glycomics analysis techniques producing interesting data. Recordings of the talks are released on the GlyGen YouTube channel.
March 28, 2023 - Rebekah L. Gundry
Department of Cellular & Integrative Physiology, College of Medicine
University of Nebraska Medical Center
Omaha, NE, USA
Expanding our View of the Cardiac Surfaceome: New Bioinformatic Tools and Technologies for Mapping Glycoproteins and Glycans from Small Sample Sizes
Cell surface glycoproteins and glycans play critical roles in a range of biological functions and disease processes. However, the lack of facile methods for sample preparation and data analysis has historically posed major challenges to their characterization. This presentation will describe the new CellSurfer and glyPAQ platforms for robust and rapid mass spectrometry-based analysis of cell surface glycoproteins and glycans and their applications to the human heart.
Webex Meeting Details
Date: Tuesday March 28, 10am ET
Meeting link: https://gwu.webex.com/gwu/j.php?MTID=m7f290a1348e2f07a418933d198d64b45
Meeting number: 2621 583 1125
Password: GlyGenTalks
February 07, 2023 - Christopher M. West
Complex Carbohydrate Research Center
University of Georgia
Athens, GA, USA
A brief excursion through the glycobiology of unicellular eukaryotes
Unicellular eukaryotes have evolved diverse adaptations to many earthly environments, including as pathogens of human and plant hosts. Their glycosylation is often similarly varied, and the extant record reflects a combination of evolutionary history and contributions to fitness that are only beginning to be explored in detail. I will introduce select aspects of the glycomics, glycogenomics and glycobiology of the soil-borne amoebozoan Dictyostelium discoideum, the apicomplexan human pathogen Toxoplasma gondii, and the agent for Chagas disease Trypanosoma cruzi.
December 06, 2022 - Lingjun Li
School of Pharmacy and Department of Chemistry
University of Wisconsin-Madison
Madison, WI, USA
Advancing Glycomics and Glycoproteomics via MultiplexedChemical Tagging Technology
Glycosylation is one of the most important and most complex protein post-translational modifications. Alterations in glycomic profiles have been linked to various diseases, including cancer, neurodegenerative disorders, and cardiovascular problems. Thus, new methods are needed for quantitative analysis of glycans to facilitate the elucidation of their diverse functional roles in human diseases.Advances in mass spectrometry (MS)-based glycoproteomics and glycomics are increasingly enabling qualitative and quantitative approaches for site-specific structural analysis of protein glycosylation. In this presentation, Iwill describe our recent efforts in developing both MS1 and MS2-based relative quantification strategies for glycomic and glycoproteomicanalysis ofbiological samples. Several cost-effective and novel isobariclabeling reagents will be highlighted for their utility in quantitativeglycanand glycopeptideanalyses ofhuman serum and cerebrospinal fluid samples.