Protein details/Glycosylation

The glycosylation section of the Protein information page in GlyGen provides the detailed list of glycosylation sites, reported glycans attached to the protein and predicted sites.

GlyGen portal

Screenshot of the Glycosylation section on the Protein information page in GlyGen.

Glycosylation is presented in 4 tabs on the Protein information page in GlyGen. The Glycosylation summary provides the overall information about the section like the total number of sites (O,N,S,C linked sites), total number of N linked and O-linked sites with the total number of glycan annotations (structures) observed on the protein.

Reported Sites with Glycan

This tab shows a table with the following columns:

• Source - GlyGen evidence linking to the databases and papers that provided the glycosylation information
• Type - Type of glycosylation. Eg. N-linked, O-linked
• GlyTouCan ID - Unique accession assigned to the registered glycan structure in GlyTouCan database. Eg. G01543ZX
• Glycan Image - Image of the glycan in SNFG format
• Residue - Amino acid residue of the given protein along with its position.
• Note - Additional information about the entry such as curation notes, O-glycosylation subtype, remarks, etc.

Source of information

The following resources provided information that was integrated in GlyGen

• UniProtKB - only reported sites information and predicted information is downloaded from UniProtKB

Data access

The Glycosylation data is collected from the resources such as UniProtKB, Glyconnect, UniCarbKB, RCSB PDB, The O-GlcNAc Database. The collected data is processed and stored in data.glygen.org in following datasets.

• Human Glycosylation Sites (UniProtKB)
• Mouse Glycosylation Sites (UniProtKB)
• Glycosylation Sites - UniCarbKB [Human proteins]
• Glycosylation sites - UniCarbKB [Mouse proteins]
• Human Glycosylation Sites (RCSB PDB)
• Mouse Glycosylation Sites (RCSB PDB)
• Glycosylation sites - UniCarbKB [Rat proteins]
• Rat Glycosylation Sites (UniProtKB)
• Rat Glycosylation Sites (RCSB PDB)
• Human Glycosylation Sites (GlyConnect)
• Mouse Glycosylation Sites (GlyConnect)
• Rat Glycosylation Sites (GlyConnect)
• HCV1a Glycosylation Sites (Literature + UniCarbKB)
• HCV1a Glycosylation Sites (UniProtKB)
• HCV1b Glycosylation Sites (UniProtKB)
• SARS-CoV2 Glycosylation Sites (UniProtKB)
• Glycosylation Sites - UniCarbKB [SARS CoV 2 proteins]
• Human Glycosylation Sites [GPTwiki]
• Human Glycosylation Sites [Automatic Literature Mining] [Automatically verified]
• SARS-CoV1 Glycosylation Sites (UniProtKB)
• Human O-GlcNAc Glycosylation Sites (MCW)
• SARS-CoV2 Glycosylation sites
• Human Glycosylation Sites UniCarbKB Glycomics Study
• SARS-CoV1 Glycosylation Sites (Literature)